The restricted access materials were immobilized on steel and platinum wires. The selective coating eliminated most of the matrix interference, which allowed the coupling to mass spectrometry without further purification. The SPME devices were interfaced to mass spectrometry by electronanospray. Several experimental set-ups are described and discussed herein. For the in situ extraction of peptides from the tryptic digests, trypsin was immobilized both on steel wires and on the inside wall of a vial. The results demonstrate that the direct coupling of SPME to nanospray can reduce analysis time and is an attractive alternative to conventional approaches like Zip-Tip purification.
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The restricted access materials were immobilized on steel and platinum wires. The selective coating eliminated most of the matrix interference, which allowed the coupling to mass spectrometry without further purification. The SPME devices were interfaced to mass spectrometry by electronanospray. Several experimental set-ups are described and discussed herein. For the in situ extraction of peptides from the tryptic digests, trypsin was immobilized both on steel wires and on the inside wall of a vial.
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PMID: On-line coupling of in-tube boronate affinity solid phase microextraction with high performance liquid chromatography-electrospray ionization tandem mass spectrometry for the determination of cis-diol biomolecules.
He J, et al. Epub Apr Nanospray 'taxation' and how to avoid it. Chernushevich IV, et al. Rapid Commun Mass Spectrom. Sajid M, et al. Crit Rev Anal Chem. Epub Dec PMID: Review. Stutz H. Show more similar articles See all similar articles.
Cited by 1 article Low invasive in vivo tissue sampling for monitoring biomarkers and drugs during surgery. Bojko B, et al. Lab Invest. Epub Mar Publication types Research Support, Non-U. Gov't Actions. Animals Actions. Cattle Actions. Molecular Sequence Data Actions. Nanotechnology Actions.
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