|Published (Last):||4 June 2016|
|PDF File Size:||16.19 Mb|
|ePub File Size:||19.30 Mb|
|Price:||Free* [*Free Regsitration Required]|
You can change your ad preferences anytime. Northern blot. Upcoming SlideShare. Like this presentation? Why not share! Embed Size px. Start on.
Show related SlideShares at end. WordPress Shortcode. Alan Carrion Follow. Published in: Technology. Full Name Comment goes here. Are you sure you want to Yes No. An eBook reader can be a software application for use on a computer such as Microsoft's free Reader application, or a book-sized computer THIS is used solely as a reading device such as Nuvomedia's Rocket eBook.
Users can purchase an eBook on diskette or CD, but the most popular method of getting an eBook is to purchase a downloadable file of the eBook or other reading material from a Web site such as Barnes and Noble to be read from the user's computer or reading device. Generally, an eBook can be downloaded in five minutes or less Browse by Genre Available eBooks Stefany Quisbert Diaz. No Downloads. Views Total views. Actions Shares.
Embeds 0 No embeds. No notes for slide. Northern blot 1. Procedimiento northern blot 4. Northern blot : transferencia por capilaridad 6. You just clipped your first slide! Clipping is a handy way to collect important slides you want to go back to later.
Now customize the name of a clipboard to store your clips. Visibility Others can see my Clipboard. Cancel Save.
Sonda de hibridación
A dot blot or slot blot is a technique in molecular biology used to detect proteins. It represents a simplification of the western blot method, with the exception that the proteins to be detected are not first separated by electrophoresis. Instead, the sample is applied directly on a membrane in a single spot, and the blotting procedure is performed. The technique offers significant savings in time, as chromatography or gel electrophoresis , and the complex blotting procedures for the gel are not required. However, it offers no information on the size of the target protein. Performing a dot blot is similar in idea to performing a western blot, with the advantage of faster speed and lower cost. Dot blots are also performed to screen the binding capabilities of an antibody.
Northern analysis remains a standard method for detection and quantitation of mRNA levels despite the advent of powerful techniques, such as RT-PCR, gene array analysis and nuclease protection assays. Northern analysis provides a direct relative comparison of message abundance between samples on a single membrane. It is the preferred method for determining transcript size and for detecting alternatively spliced transcripts. The Northern blotting procedure is straightforward and provides opportunities to evaluate progress at various points e. RNA samples are first separated by size via electrophoresis in an agarose gel under denaturing conditions. The RNA is then transferred to a membrane, crosslinked and hybridized with a labeled probe.